QTL-seq¶
Bulked segregant analysis, as implemented in QTL-seq (Takagi et al., 2013), is a powerful and efficient method to identify agronomically important loci in crop plants. QTL-seq was adapted from MutMap to identify quantitative trait loci. It utilizes sequences pooled from two segregating progeny populations with extreme opposite traits (e.g. resistant vs susceptible) and a single whole-genome resequencing of either of the parental cultivars. While the original QTL-seq algorithm did not assume a highly heterozygous genome, a “modified QTL-seq” has been developed to handle this situation using high resolution mapping (Itoh et al., 2019)
Environment Modules¶
Run module spider QTL-seq to find out what environment modules are available for this application.
System Variables¶
- HPC_QTLSEQ_DIR - installation directory
- HPC_QTLSEQ_BIN - executable directory