Olego¶
OLego is a program specifically designed for de novo spliced mapping of mRNA-seq reads. OLego adopts a seed-and-extend scheme, and does not rely on a separate external mapper. It achieves high sensitivity of junction detection by strategic searches with very small seeds (12-14 nt), efficiently mapped using Burrows-Wheeler transform (BWT) and FM-index. This also makes it particularly sensitive for discovering small exons. OLego is implemented in C++ with full support of multiple threading, to allow for fast processing of large-scale data.
Environment Modules¶
Run module spider Olego to find out what environment modules are available for this application.
System Variables¶
- HPC_OLEGO_DIR - installation directory
- HPC_OLEGO_BIN - executable directory