Metilene¶
metilene is a software tool to annotate differentally methylated regions (DMRs) and differentially methylatedcytosine sites (DMCs) from Methyl-Seq data. metilene accounts for intra-group variances and offers differentmodes de-novo DMR detection, DMR detection within a known set of genomic features, and DMC detection.Various biological data can be used, metilene works for Whole-Genome Bisulfite Sequencing (WGBS), Re-duced Representation Bisulfite Sequencing (RRBS), and any other input data, as long as absolute (methyla-tion) levels and genomic coordinates are provided. metilene uses a circular binary segmentation and a 2D-KStest to call DMRs. Adjusted p-values are calculated using the Bonferroni or Benjamini-Hochberg correction(see option-c,--mtc).
Environment Modules¶
Run module spider Metilene to find out what environment modules are available for this application.
System Variables¶
- HPC_METILENE_DIR - installation directory