FastQCFastQC Report
Tue 11 Oct 2022
CBh_codebook_S60_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBh_codebook_S60_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503515
Sequences flagged as poor quality0
Sequence length101
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGCTGCCGACGAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT29450.5848882357030079Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCCGACCAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT25820.5127950507929258Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCCGACGAGTACTGACGTGTAGATCTCGGTGGTCGCCGTATCAT23970.47605334498475715Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCAGAACAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT17090.3394139201414059Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCAGAACAGTACTAACGTGTAGATCTCGGTGGTCGCCGTATCAT15920.31617727376542903Illumina RNA PCR Primer (96% over 32bp)
GACGCTGCAGACCAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT15100.2998917609207273Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCAGACGAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT14630.29055738160730066Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCCGACCAGTACTGACGTGTAGATCTCGGTGGTCGCCGTATCAT13830.2746690763929575Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCAGAAGAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT12600.2502408071259049Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCCGAACAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT12330.24487850411606407Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCCGAAGAGTACTTACGTGTAGATCTCGGTGGTCGCCGTATCAT11260.22362789589188006Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCCGACCAGTACTAACGTGTAGATCTCGGTGGTCGCCGTATCAT11250.2234292920767008Illumina RNA PCR Primer (96% over 32bp)
GACGCTGCAGACCAGTACTAACGTGTAGATCTCGGTGGTCGCCGTATCAT8340.16563558185952754Illumina RNA PCR Primer (96% over 32bp)
GACGCTGCCGAACAGTACTAACGTGTAGATCTCGGTGGTCGCCGTATCAT7480.14855565375410862Illumina RNA PCR Primer (96% over 32bp)
GACGCTGCAGACGAGTACTGACGTGTAGATCTCGGTGGTCGCCGTATCAT6950.13802965154960628Illumina RNA PCR Primer (100% over 30bp)
GACGCTGCCGACGAGTACTAACGTGTAGATCTCGGTGGTCGCCGTATCAT6200.12313436541115953Illumina RNA PCR Primer (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5620.11161534413076075No Hit
GACGCTGCCGAAGAGTACTGACGTGTAGATCTCGGTGGTCGCCGTATCAT5200.1032739838932306Illumina RNA PCR Primer (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC5750.094.920766
AGCCGGC254.63986E-794.920763
ACGCCAA254.63986E-794.920766
TTGTGGA1000.094.920763
TTCAGCA254.63986E-794.920763
CATGCAC1350.094.920764
CGTGCCC550.094.920764
GACACCG8100.094.920768
GACACAT254.63986E-794.920766
GGCACAT4700.094.920766
CGGCACA2200.094.920765
AAAAGGA550.094.920763
TCGATGC254.63986E-794.920762
TCGGCCC550.094.920764
GCCGCCC500.094.920764
AACGGCA254.63986E-794.920763
GCACAAT3050.094.920767
TCTCGCA254.63986E-794.920763
CGCAGCA254.63986E-794.920763
GATGCCC1100.094.920764
Produced by FastQC (version 0.11.4)