FastQCFastQC Report
Tue 11 Oct 2022
CBh_codebook_S60_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBh_codebook_S60_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503515
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG64221.2754337010813976No Hit
GACATATGAAGTGCTCTAGTCAAGTTTAACTGGTGTCCACAGAGGACATG8970.17814762221582278No Hit
ATACTGCACTGTACACTCTGACATATGAAGTGCTCTAGTCAAGTTTAACT6050.1201553081834702No Hit
GACATATGAAGTCCTCTAGTCAAGTTTAACTGGTGTCCACAGAGGACATG6020.11955949673793233No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGA156.609395E-495.022961
GAAGCGG156.6198467E-494.98529
GCGAAGC254.6211426E-794.98527
CGTTCGC156.6198467E-494.98529
GTAGCGG156.6198467E-494.98529
AGCATCG156.6198467E-494.98528
CCTATCG301.2318196E-894.98528
GATGCGC750.094.985199
TTAAGGC156.63031E-494.947473
TTAGTCG850.094.947475
CGGTAGT353.3105607E-1094.947473
GCGTAGT301.2352757E-894.947473
GTATAGC201.7476676E-594.9474645
GTGGCTA37400.094.3502668
ACAATCG9200.092.92038
AAGTGGC13950.092.905596
AGTGGCT38700.092.7762457
GGGGACA11350.092.437844
CGAGTGG1700.092.15495
GGGGGAC14450.091.990623
Produced by FastQC (version 0.11.4)