FastQCFastQC Report
Tue 11 Oct 2022
A744_Heart_S30_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA744_Heart_S30_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACACATTGAACCGGAGATTAGACAGGGTCTAGTCTCAAGAACATAAA17.142857142857142No Hit
AAAAGTGTATCAACCACCGATAAAGAAACTACCAATTAAGATAGCCACTA17.142857142857142No Hit
AAAATAGCAAGTATCAAAGAAACAAATACTAGAAAGGAAGAGCGTCGTGT17.142857142857142No Hit
CTATTAGTCTCGTCCGGTGGGAACTCAGGGTCGGTTATTTTATACATAAA17.142857142857142No Hit
CTTAAGGGCCAGGGCACGGGAAGCTAAACAGTGATAGAAGGTGGTGCATA17.142857142857142No Hit
TGACAGTCAACACGCAGCTTACCATTTATGTGTGGTGGTGCAGATGAACT17.142857142857142No Hit
TTTCTTGACTCCTCCGGAGGTAACTCAGGGTCGTCCGTAGTCTACATAAC17.142857142857142No Hit
ATTGGTGCCCAACGCACCGAAAGCTTACCAATGATTGTGGGTGGTGCAGA17.142857142857142No Hit
GACAGGGGACAGGGCACGGGAAGCTTACCCGATTGTCGAGGTGGTGCAGA17.142857142857142No Hit
GGTCTTGCCCACAATGAAATCCACTGAAACTAGATCGGAGAGCGTCGTGT17.142857142857142No Hit
GGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17.142857142857142No Hit
GTAGATGACTCTACCGGAGTTAACTCAGGGTCTGATGTCATGTACATAAA17.142857142857142No Hit
AATAATGTAACTTCCACCGTAAACTAACCCAATGGAATATATTTAAAAAA17.142857142857142No Hit
AGGTATGGACAGAATGAAACACACTCCGTTGAGATCGGAAGAGCGTCGTG17.142857142857142No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.4)