FastQCFastQC Report
Tue 11 Oct 2022
A744_Gastroc_S25_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA744_Gastroc_S25_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGTGCACAATATGAAAAACACTAGACACAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
TAAATGGGCCAGCCCAGAATTATAGCCACTTACCCCAGATCGGAAGAGCG13.225806451612903No Hit
ATCCGGGGACATTATGAAAGCCACTAATAAAAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
TGTAAAGGACACCTAAAATAAAACAAAAAATATATATTAAGAGGTTCTTT13.225806451612903No Hit
GTCCGGCAATCCTCCGGAGCTAACTCAGGGTCTGTGCGTTTCTACATAAA13.225806451612903No Hit
ATATTTGGCCAAAATGAAACCCACTTGATAAAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
GGACAAGGCCATTATGATAGCCACTTAGTCGAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
TCATAAGACAAAAATGAAACAAACTCAATTGAGATCTGAAGACCGTAGTT13.225806451612903No Hit
AAAAAAGACAAAACCACAGAAAACTAACCTTAACTTATTTGATAAAAAAA13.225806451612903No Hit
AAAATGGAAAATAAAGAAACAAACTAACAATATATCTCAAGATACAATAC13.225806451612903No Hit
TTTTCTGGCCAGAATGATATACACTGGTACAAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
CTAACTGAAACCACCACTGAAAACTAACCGTAAATGTAAGTTTAAAAAAA13.225806451612903No Hit
TCCATGGCACACAATGAAAAACACTAAAAAAAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
TATAAAGAAAACACCGCAGCCAACTAAGGTGAAATATGCAGATACATAAA13.225806451612903No Hit
AATCGAGCCCAACGCCACTATGATAGCCACTTCGCGAAGATCGGAAGAGC13.225806451612903No Hit
TCGTGGGGACACAATGAAACCCACTGATAAAAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
TTTCAGGGACAGGGCACGGGAAGCTTACCTACCACCCTTGGTGGTGCAGA13.225806451612903No Hit
CGGTTAGGCCATGGCACGGGAAGCTTACCAGGCGGTGTCGGTGGTGCAGA13.225806451612903No Hit
GTTAAGGGCCATGGCACGGGAAGCTTACCAAGGATCAACGGTGGTGCAGA13.225806451612903No Hit
GGACTGGACTCTACCGGTGTTAACTCAGGGTCGCCCTCTGTCTACATAAA13.225806451612903No Hit
ACTAACGTCAATTAAGTTACAAAATAATGTAATATCCAAAAAGATACCAT13.225806451612903No Hit
CAGTGTGGACAGGGCACGGGCAGCTTACCGTTTAGAATTGGTGGTGCAGA13.225806451612903No Hit
GTGCAAGCACATTATGAAAGACACTTCCCACAGATCGGAAGAGCGTCGTG13.225806451612903No Hit
TATATCGCACAACGCACGGAAAGCTTACCAATGTCTAAAGGTGGTGCAGA13.225806451612903No Hit
AAGTTGGAATCGTCCGGAGGTAACTCAGGGTCGCTTGTGCAATACATAAA13.225806451612903No Hit
TCAAATGCCCACCGCACCGACAGCTTACCTGCCGCTTTGGGTGGTGCAGA13.225806451612903No Hit
CTGAATGTATCGTCCGGAGGTAACTCAGGGTCGGAGCTGTTTTACATAAA13.225806451612903No Hit
GGCTAAGCACATAATGATAAACACTAGCGTGAGATCGGAAGAGCGTAGTG13.225806451612903No Hit
GTGGTGGGATCTTTAGGTGTTAACTACGGGGATTACCGATGGTAGATAAA13.225806451612903No Hit
AATAAAATCAATTAAGATATCAACTAAAAAAATATAGAAATATACAAAAC13.225806451612903No Hit
AACTACGCACATGGCACGGCCAGCTTACCTTAAACGTTTGGTGGTGCAGA13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.4)