FastQCFastQC Report
Tue 11 Oct 2022
A744_Gastroc_S25_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA744_Gastroc_S25_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATAACTAGAAAAAAAACACAATATTATCAGGTGAGCAAGGGCGAGGA13.225806451612903No Hit
AAATTGAAAGTCTAACAAACAACCAAAACTCAGATAGGAACGGCCCACGA13.225806451612903No Hit
TGATGTAGTGGATATCATAATGAATATTATCAAGTGAGCGAGGGCGAGGA13.225806451612903No Hit
TGGATCAGTCCATATAATAATCTCAGTTAGTCTCTTTCCCGTGGTACGTG13.225806451612903No Hit
GTGTCTAATTAAAAAAAAAAAATACACGCTAAGATCGGAAGAGCACACGT13.225806451612903No Hit
TTATCAACTGGCTATCATACTCGCCAAATATAGATCGGAAGAGCACACGT13.225806451612903No Hit
CCGGTCATAGCAAAACAAAAACCCTATTATCAGGTGAGCAAGGGCGAGGA13.225806451612903No Hit
AAACACAGAAACAATAAAAATTTCTATTATCAGCAGACCGTCATAAGCGA13.225806451612903No Hit
ACAATGACTGTCTATCAAACAGCCTATTATCAGCTTGCGATAGTGGATTA13.225806451612903No Hit
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG13.225806451612903No Hit
CGGTTTACTTGATATAAAACATTCTATTATCAGCCGACCGTCATTAGCGC13.225806451612903No Hit
TTTAAAAGTGTATATCATAATTTCTATTATCAGAACGGAAGAGGACTCGT13.225806451612903No Hit
TGTACCATTGGCTATAATAATACACAGAAAAAGATCGGAAGAGCACTCGT13.225806451612903No Hit
GGGGTAAGTGCCAATCATACTGACCTGGCCCATTTAAGATCGGAAGAGCA13.225806451612903No Hit
TCGCGAACACCAAAACAAACACACCCTGGCCTCGATTAGATCGGAAGAGC13.225806451612903No Hit
TATCACATTGCATAAAAAAATCACTATTATCAGCCGACCGACTTTAGCGC13.225806451612903No Hit
AAAAAGACACGATAAAATAAACTATATTATCAGAAAGCACAGTACACTGT13.225806451612903No Hit
CAATTGAGTGGCTATAAAAATAAACATATGAAGATCGGAAGTGCACACCT13.225806451612903No Hit
GAAGTTACTGGCAAAAAAAAACCCTATTATCAGGTGAGCAAGGGCGAGGA13.225806451612903No Hit
AGAATAACAAAAAAACAAAAAAAAAAAAAACAGAACACAATAAAAAGCGC13.225806451612903No Hit
AAAGTCAGTGGCTATCATACTCGCTATTATCAGCCGACCGTCATTAGCGC13.225806451612903No Hit
ATTTTAACACTCTATCATACTGACAAAAAGTAGAAAGCAAGTGAACAGGT13.225806451612903No Hit
AAGATTATTGCCTATCAAAATGTCTATTATCAGGTGAGCAAGGGCGAGGA13.225806451612903No Hit
TTTATCAGTGGCTATCATACTGGACCCACGAAGATCGGAAGAGCACACGT13.225806451612903No Hit
AAAAACAGTTGCTATCATACTGTATATTATCAGGTGAGCAAGGGCGAGGA13.225806451612903No Hit
GTGGGAAGTGCCTATCAAACACTCCTTGCACAGATCGGAAGAGCACACGT13.225806451612903No Hit
AATGTAAATATCAAAAAAACAAACTATTATCAGCCGACCGTCATTAGCGC13.225806451612903No Hit
TTTATTAATAACTATCAAAATCCACCCGGATAGATCGGAAGAGCACACGT13.225806451612903No Hit
TTTTTTAATACCAAAAAAAAACACCCATGGAAGATCGGAAGAGCACACGT13.225806451612903No Hit
CACGCTACAACCTATCAAACTTGACTTAGCCAGATCGGAAGAGCACACGT13.225806451612903No Hit
CGACTAAGTGGCTATCATACTGGCCTTGTCCAGATCGGAAGAGCACACGT13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.4)