FastQCFastQC Report
Tue 11 Oct 2022
A743_TA_S20_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA743_TA_S20_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849463
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATC2650.094.908927
GTGCACT409.094947E-1294.908915
GCGACAT1050.094.908916
CATGCGC156.644227E-494.908912
CATGCAC10850.094.908914
ACGCAGC550.094.908912
CGCGCCC1300.094.908914
GATCAGC301.2409146E-894.908912
GCGTTCC156.644227E-494.908912
ATGTCAC254.6489185E-794.908914
AGTATGC2250.094.908912
ATGTAGC800.094.908912
ATCAAGC700.094.908912
ATTCCGC201.7526023E-594.908912
AGCGGAC650.094.908914
GCACACT28600.094.908917
GCACAAC12500.094.908917
CGTAGAC156.644227E-494.908914
AAACCGC201.7526023E-594.908912
AATCTGC500.094.908912
Produced by FastQC (version 0.11.4)