FastQCFastQC Report
Tue 11 Oct 2022
A743_TA_S20_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA743_TA_S20_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849463
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG30290.10630073105002591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCA254.616104E-795.0218661
GTGCTTA500.095.0218661
GATGCGA650.095.0218661
GGTTCGA450.095.0218661
GTTGGTA1250.095.0218661
TCGGTTA1050.095.0218661
GGATCGA750.095.021861
GATGGTA850.095.021861
GTCGTTA850.095.021861
AGTTTCT12300.094.995187
ATGGGAT450.094.9818342
TTAGGAT450.094.9818342
ATGTGAT353.3105607E-1094.9818342
TCGGGAT353.3105607E-1094.9818342
AAGGTAC1000.094.9818342
AGGGTAT700.094.9818342
TAGTTAT1150.094.9818342
GATGGAT500.094.9818342
AGCGAAC353.3105607E-1094.9818342
GGTGCAT254.627691E-794.9818342
Produced by FastQC (version 0.11.4)