FastQCFastQC Report
Tue 11 Oct 2022
A743_Heart_S31_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA743_Heart_S31_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447409
Sequences flagged as poor quality0
Sequence length101
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC353.3287506E-1094.892052
GGAGCAC353.3287506E-1094.892054
ATTAGCC700.094.892053
TCATTGC353.3287506E-1094.892052
GCTGGGA353.3287506E-1094.892053
AGCCCAT1950.094.892056
GCTTGGA700.094.892053
CGCACAT700.094.892056
TTACAGC353.3287506E-1094.892052
TTCGACA353.3287506E-1094.892055
GTAAAGC353.3287506E-1094.892052
GCCCCAC254.6473724E-794.8920446
TTCTAGC301.2400051E-894.8920442
GCCCATT1350.094.8920447
GCCCACT1800.094.8920447
GAAGCAC1050.094.8920444
GCGACAC156.6452275E-494.8920446
CAAGTGC301.2400051E-894.8920442
TGCCACG254.6473724E-794.8920447
GCGAATC156.6452275E-494.8920445
Produced by FastQC (version 0.11.4)