FastQCFastQC Report
Tue 11 Oct 2022
A743_Gastroc_S26_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA743_Gastroc_S26_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055058
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGC550.094.9127042
ATCCTGC301.2398232E-894.9127042
GCCTTGC550.094.9127042
CATGCTC156.642002E-494.9127044
CGCGGAC550.094.9127044
ATGGCCT600.094.9127045
GTGGGCT4200.094.9127045
AACCAGC254.6457717E-794.9127042
GATCCTG156.642002E-494.9127041
GCTATGC600.094.9127042
GATCCAC353.3287506E-1094.9127044
CGCGCCC353.3287506E-1094.9127044
CCAGCAC700.094.9127044
GACCCGC156.642002E-494.9127042
CATTAGC353.3287506E-1094.9127042
GTTACTG254.6457717E-794.9127041
TGCATGC550.094.9127042
ACGATGC353.3287506E-1094.9127042
AGCGGAC254.6457717E-794.9127044
AATGCAC7500.094.9127044
Produced by FastQC (version 0.11.4)