FastQCFastQC Report
Tue 11 Oct 2022
A743_Gastroc_S26_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA743_Gastroc_S26_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055058
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG15810.14984958172915613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTA1100.095.020921
GTGTTGA550.095.020921
GTCATGA254.6143396E-795.020921
AGCGTTA254.6143396E-795.020921
CGTGGGA156.6119846E-495.020921
TGGAGGA301.2300006E-895.020921
GAGCTTA254.6143396E-795.020921
GGTCGCA156.6119846E-495.020921
GGTCGAA156.6119846E-495.020921
GGCACGA156.6119846E-495.020921
GGTCCTA156.6119846E-495.020921
GCGTTGA254.6143396E-795.020921
AGCGGTA254.6143396E-795.020921
AGCGGGA156.6119846E-495.020921
TTCCCGA156.6119846E-495.020921
TCGTGGA301.2300006E-895.020921
GTGCTTA254.6143396E-795.020921
GTTGTCA600.095.020921
GTTCGCA301.2300006E-895.020921
GCGGGTA254.6143396E-795.020921
Produced by FastQC (version 0.11.4)