FastQCFastQC Report
Tue 11 Oct 2022
A742_TA_S22_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA742_TA_S22_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950571
Sequences flagged as poor quality0
Sequence length101
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC156.6478347E-494.8910142
GTACCAC254.651738E-794.8910144
AGCCCAA5500.094.8910146
GCTATGC500.094.8910142
TGGCGAC409.094947E-1294.8910144
GATCCGC201.7536406E-594.8910142
GCGTTGC409.094947E-1294.8910142
TCGGCCC1000.094.8910144
TAGCAGC254.651738E-794.8910142
ATCCGCC201.7536406E-594.8910143
AGTGCAA201.7536406E-594.8910144
GCACAAT5150.094.8910147
TCCAGAC156.6478347E-494.8910144
GTCACAC201.7536406E-594.8910146
ACGTGAC156.6478347E-494.8910144
AATCTGC353.3287506E-1094.8910142
ATCCATG301.2416422E-894.8910141
AACTGCG156.6478347E-494.8910141
GATGCGC201.7536406E-594.8910142
GCGATGC254.651738E-794.8910142
Produced by FastQC (version 0.11.4)