FastQCFastQC Report
Tue 11 Oct 2022
A742_TA_S22_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA742_TA_S22_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950571
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG12390.13034270980284482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTA550.095.047191
AGGTGTA750.095.047191
TCTGCAA156.6045224E-495.047181
TTGATGA650.095.047181
AGCGTTA254.6064088E-795.047181
CGTGGGA201.7393802E-595.047181
GGTACGA301.22745405E-895.047181
GAAGCGA156.6045224E-495.047181
ATGATGA650.095.047181
CATGCGA156.6045224E-495.047181
CCGATGA156.6045224E-495.047181
TTTGTTA600.095.047181
TTTGTCA353.2923708E-1095.047181
TGGGTTA650.095.047181
GTCCTCA156.6045224E-495.047181
TTTCGCA254.6064088E-795.047181
TATGGGA409.094947E-1295.047181
TGTGGGA500.095.047181
TGTGGCA353.2923708E-1095.047181
TCGGGCA156.6045224E-495.047181
Produced by FastQC (version 0.11.4)