FastQCFastQC Report
Tue 11 Oct 2022
A742_Heart_S32_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA742_Heart_S32_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424729
Sequences flagged as poor quality0
Sequence length101
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCCC1250.094.8887564
TCTGCCC1500.094.888754
TGTCACA156.6459115E-494.888755
ATGGGAC2150.094.888754
GCCCATC2050.094.888757
ACAGTGC156.6459115E-494.888752
TGCCACC156.6459115E-494.888757
CGGCCCA1350.094.888755
TCACAAT156.6459115E-494.888757
TCCTGCA301.240187E-894.888753
CATGAAT156.6459115E-494.888754
TTGTCTC156.6459115E-494.888755
TGGCGAC156.6459115E-494.888754
TCCCTGC156.6459115E-494.888752
AGGACAT5550.094.888756
GATCATG156.6459115E-494.888751
AGTGGAC2700.094.888754
TACGGGA301.240187E-894.888753
TGCGGGA156.6459115E-494.888753
TATGCAC4400.094.888754
Produced by FastQC (version 0.11.4)