FastQCFastQC Report
Tue 11 Oct 2022
A742_Heart_S32_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA742_Heart_S32_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424729
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG5740.13514499834011806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGA450.095.059881
TTGCTTA450.095.059881
GTTGTGA450.095.059881
TTGGTTA450.095.059881
GGGTTTA450.095.059881
TGTGTTA450.095.059881
GTGTTTA353.274181E-1095.0598751
AACGTTA201.7371132E-595.0598751
TTAGGTA409.094947E-1295.0598751
ATGGGGA201.7371132E-595.0598751
GGTACGA156.5984775E-495.0598751
GGTACCA156.5984775E-495.0598751
TCACCGA156.5984775E-495.0598751
GGGCTTA156.5984775E-495.0598751
GGTCGTA156.5984775E-495.0598751
TTGTGGA201.7371132E-595.0598751
ACGTTTA201.7371132E-595.0598751
GCGGTGA156.5984775E-495.0598751
TTTGTTA409.094947E-1295.0598751
GCGCGAA156.5984775E-495.0598751
Produced by FastQC (version 0.11.4)