FastQCFastQC Report
Tue 11 Oct 2022
A742_Gastroc_S27_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA742_Gastroc_S27_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840338
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATC1800.094.900047
GCGACAT353.3287506E-1094.900046
GTCGAGC201.7526936E-594.900042
CGCGGAC353.3287506E-1094.900044
GATCCAC201.7526936E-594.900044
TCGGGAC353.3287506E-1094.900044
CGCGCAC201.7526936E-594.900044
AGTGGAC7400.094.900044
ATACTGC409.094947E-1294.900042
GTCAGCC201.7526936E-594.900043
GGCCAAT8500.094.900047
AATGCAC7450.094.900044
GCACAAT15600.094.900047
TTCCAGC201.7526936E-594.900042
GGGAATC353.3287506E-1094.900045
AATTGCG201.7526936E-594.900041
TACTGCC850.094.900043
CGGTGCC201.7526936E-594.900043
GACGGAC450.094.900044
GCGATGC353.3287506E-1094.900042
Produced by FastQC (version 0.11.4)