FastQCFastQC Report
Tue 11 Oct 2022
A742_Gastroc_S27_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA742_Gastroc_S27_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840338
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG14620.17397761376969742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA254.611793E-795.0269241
AGGGGTA254.611793E-795.0269241
AAAGGGA254.611793E-795.0269241
GTTGGGA500.095.0269241
AGCGTTA301.2290911E-895.026921
ATGGGCA201.7411045E-595.026921
GGTCGTA353.2923708E-1095.026921
GCGGTTA353.2923708E-1095.026921
TCTTCGA409.094947E-1295.026921
CGTTGGA156.609852E-495.026921
TCGGGCA156.609852E-495.026921
GTAGGGA301.2290911E-895.026921
AGGCGAA201.7411045E-595.026921
GCGTTGA156.609852E-495.026921
GCGTTCA201.7411045E-595.026921
TCGGCAA301.2290911E-895.026921
GTGTGGA409.094947E-1295.026921
AGCGGCA156.609852E-495.026921
GAGGTTA201.7411045E-595.026921
AGGTTCA353.2923708E-1095.026921
Produced by FastQC (version 0.11.4)