FastQCFastQC Report
Tue 11 Oct 2022
A741_TA_S23_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA741_TA_S23_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136398
Sequences flagged as poor quality0
Sequence length101
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC13600.094.893966
GCCCACT3950.094.893967
GCGCGCA409.094947E-1294.893963
CAGTTGC450.094.893962
TAGCGGA156.6473515E-494.893963
TCCCTGC201.7535185E-594.893962
GCCGGCA301.2416422E-894.893963
CATCTGC201.7535185E-594.893962
TGACGCC301.2416422E-894.893963
GACTTGC450.094.893962
ATCCGCC156.6473515E-494.893963
GCTCTGC201.7535185E-594.893962
GCCGACA301.2416422E-894.893965
GCCTGCC450.094.893963
GCCTGCA700.094.893963
TATCTGC353.3287506E-1094.893962
AGTGAGC409.094947E-1294.893962
GAGCGGA201.7535185E-594.893963
ACGGAGC201.7535185E-594.893962
AGCGAGC201.7535185E-594.893962
Produced by FastQC (version 0.11.4)