FastQCFastQC Report
Tue 11 Oct 2022
A741_TA_S23_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA741_TA_S23_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136398
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG14830.13050005367837675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGA650.095.034621
CTTCGGA156.608329E-495.034621
TTTCGTA600.095.034621
TAGGTTA600.095.034621
CGCGCGA156.608329E-495.034621
GGATCCA156.608329E-495.034621
GTAGGGA650.095.034621
GTTACGA156.608329E-495.034621
TTTGCGA301.2289092E-895.034621
AGTAGGA156.608329E-495.034621
AGCGTTA500.095.0346151
GAAGCGA254.6106106E-795.0346151
GATGTGA500.095.0346151
GGATGTA353.2923708E-1095.0346151
GTTTCGA353.2923708E-1095.0346151
AATGGGA409.094947E-1295.0346151
CGTTCTA500.095.0346151
GGGCGTA254.6106106E-795.0346151
TTCGGTA409.094947E-1295.0346151
TAAGGTA750.095.0346151
Produced by FastQC (version 0.11.4)