FastQCFastQC Report
Tue 11 Oct 2022
A741_Heart_S33_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA741_Heart_S33_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391626
Sequences flagged as poor quality0
Sequence length101
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAC1850.094.892614
TTGATGC254.6461173E-794.892612
GTTGAAG201.7522005E-594.892611
GAAATGC254.6461173E-794.892612
GACAGTA2450.094.892618
ATGGGAC2150.094.892614
GAGTGCA409.094947E-1294.892613
AGCCGCC201.7522005E-594.892613
TTGGCAC3850.094.892614
ACAAAGC353.3287506E-1094.892612
TCCGCAC500.094.892614
ATTAGCC900.094.892613
ATAGGAC2350.094.892614
GTTTCGA201.7522005E-594.892613
ATCAGCA201.7522005E-594.892613
GCTATGC201.7522005E-594.892612
TCGGGAC450.094.892614
TATATGC700.094.892612
TGTATGC254.6461173E-794.892612
ATATGCC800.094.892613
Produced by FastQC (version 0.11.4)