FastQCFastQC Report
Tue 11 Oct 2022
A741_Heart_S33_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA741_Heart_S33_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391626
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG5300.13533320055358938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGA409.094947E-1295.0561141
TTTAGGA156.599128E-495.0561141
AACGTGA156.599128E-495.0561141
GATGTTA301.2247256E-895.0561141
AGTTTGA301.2247256E-895.0561141
TTGTGGA254.5987326E-795.0561141
ACGTTGA156.599128E-495.0561141
TTTGTGA301.2247256E-895.0561141
TTGCTTA353.274181E-1095.0561141
TTTGTCA201.737285E-595.0561141
ATGTGTA156.599128E-495.0561141
TATGGGA201.737285E-595.0561141
TAGGTAA254.5987326E-795.0561141
TGTGGGA156.599128E-495.0561141
TTGTCCA156.599128E-495.0561141
GGTCCGA156.599128E-495.0561141
GCCGGAA156.599128E-495.0561141
ATGCTTA500.095.0561141
TGTATGA201.737285E-595.0561141
AGTGGTA254.5987326E-795.0561141
Produced by FastQC (version 0.11.4)