FastQCFastQC Report
Tue 11 Oct 2022
A741_Gastroc_S28_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA741_Gastroc_S28_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802074
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATC600.094.909545
GGGCTCA156.6422974E-494.909545
ATGTCTC301.2396413E-894.909545
ACGTATC301.2396413E-894.909545
AGGTATC450.094.909535
GTGAATC201.7517741E-594.909535
TTGCGGA750.094.9036253
AGGCTGT1500.094.9036257
ATCCTGC409.094947E-1294.903622
ATCCTCC800.094.903629
TATCAGC409.094947E-1294.903622
GAAATGC600.094.903622
GGCAGGA301.240187E-894.903623
CCTTAGC156.6439435E-494.903622
GCAAGTC156.6439435E-494.903627
GCCCAGC4250.094.903627
GCGACAC201.7523165E-594.903626
ATGTGCC1100.094.903623
GCTATGC850.094.903622
GCAGTGC156.6439435E-494.903622
Produced by FastQC (version 0.11.4)