FastQCFastQC Report
Tue 11 Oct 2022
A741_Gastroc_S28_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA741_Gastroc_S28_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802074
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG13970.17417345531709044No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA201.7412567E-595.024711
CGGGTCA254.6122295E-795.024711
GGTACGA156.610352E-495.024711
GAAGCGA254.6122295E-795.024711
TCTTCGA156.610352E-495.024711
TATGGGA201.7412567E-595.024711
TAGCGGA156.610352E-495.024711
GTGATGA409.094947E-1295.024711
GTTACGA254.6122295E-795.024711
GCTTCGA156.610352E-495.024711
AGGTTCA301.229273E-895.024711
TCGCTTA409.094947E-1295.024711
CGCAGGA254.6122295E-795.024711
ATGCGCA156.610352E-495.024711
CGATGGA156.610352E-495.024711
TTGCCGA156.610352E-495.024711
TAGGCGA156.610352E-495.024711
ATACGGA301.229273E-895.024711
GCGCTTA156.610352E-495.024711
GTTGGGA301.229273E-895.024711
Produced by FastQC (version 0.11.4)