FastQCFastQC Report
Tue 11 Oct 2022
A740_TA_S24_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA740_TA_S24_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196966
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC156.6482805E-494.890942
GCTATGC450.094.890942
CCCAGCA450.094.890943
CATCTGC156.6482805E-494.890942
TGACGCC254.652502E-794.890943
CAGGCCC1950.094.890944
AACGACA301.2418241E-894.890945
GATGCAC3600.094.890944
CGTACGC156.6482805E-494.890942
GAACCGC301.2418241E-894.890942
CGCACAT2600.094.890946
TTCGCGC156.6482805E-494.890942
AAGCTCC156.6482805E-494.890943
ATCTGCC600.094.890943
GCTTTGC500.094.890942
GCCCATA5900.094.890937
GTCGCAC1100.094.890934
GGAGCAC1600.094.890934
ATCTACC950.094.890939
TGCATGC409.094947E-1294.890932
Produced by FastQC (version 0.11.4)