FastQCFastQC Report
Tue 11 Oct 2022
A740_TA_S24_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA740_TA_S24_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196966
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG13040.10894210863132286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA800.095.037761
GTTTGCA409.094947E-1295.037761
AGCGTTA201.7404225E-595.037761
CGTGGTA450.095.037761
CGGGTCA201.7404225E-595.037761
TTAGGGA500.095.037761
AATCCGA301.2285454E-895.037761
GGTACGA201.7404225E-595.037761
GAAGCGA156.6075486E-495.037761
CGAACGA156.6075486E-495.037761
GGTGTTA600.095.037761
TTGTGCA201.7404225E-595.037761
CGCGGTA201.7404225E-595.037761
GCGGTGA353.2923708E-1095.037761
TTTCGTA500.095.037761
TAGGTTA750.095.037761
TTTCGGA450.095.037761
GGCGTTA301.2285454E-895.037761
TGTGGGA500.095.037761
TTCGTTA550.095.037761
Produced by FastQC (version 0.11.4)