FastQCFastQC Report
Tue 11 Oct 2022
A740_Heart_S34_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA740_Heart_S34_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501601
Sequences flagged as poor quality0
Sequence length101
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCC750.094.884183
GCCCAGC6700.094.884187
ATCTACC750.094.884189
GGCACAC7400.094.884186
TCAGCAC1700.094.884184
ATTGGCA750.094.884183
AAAGGGA850.094.884183
AGAGGAC850.094.884184
ACTGGGA550.094.884183
GCCCCAA254.650501E-794.884176
ATCCTCC1000.094.884179
AGGTAGC201.753457E-594.884172
GAGTGCA409.094947E-1294.884173
GCCCATA900.094.884177
ACAGTGC201.753457E-594.884172
GATGTGC201.753457E-594.884172
ATCTAGC409.094947E-1294.884172
TTGTGGA2050.094.884173
AAGAGGC409.094947E-1294.884173
TCACACC409.094947E-1294.884177
Produced by FastQC (version 0.11.4)