FastQCFastQC Report
Tue 11 Oct 2022
A740_Heart_S34_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA740_Heart_S34_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501601
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG5210.1038674165322637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA353.2923708E-1095.052991
TTAGGGA201.7380471E-595.052991
AGTTTGA409.094947E-1295.052991
CACGGTA201.7380471E-595.052991
GGATGTA353.2923708E-1095.052991
TTTACCA201.7380471E-595.052991
ACTTGTA201.7380471E-595.052991
CTTTTGA201.7380471E-595.052991
TAGGTTA201.7380471E-595.052991
TATGGGA353.2923708E-1095.052991
CGGTTCA201.7380471E-595.052991
TCCCTTA201.7380471E-595.052991
ATTCGTA353.2923708E-1095.052991
AGGGTTA201.7380471E-595.052991
AGTGGTA201.7380471E-595.052991
AGGCGTA201.7380471E-595.052991
CTGGTTA201.7380471E-595.052991
TGTTGTA409.094947E-1295.052991
GCTTCGA201.7380471E-595.052991
CTAGTTA201.7380471E-595.052991
Produced by FastQC (version 0.11.4)