FastQCFastQC Report
Tue 11 Oct 2022
A740_Gastroc_S29_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA740_Gastroc_S29_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876075
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC254.6474815E-794.9045642
AGTGGCA1000.094.9045643
TGCGGCA409.094947E-1294.9045643
GCGTTGC409.094947E-1294.9045642
CACCTGC201.7523202E-594.9045642
ATTCCGC254.6474815E-794.9045642
AGTGCGC254.6474815E-794.9045642
ATTCAGC450.094.9045642
CAGATGC201.7523202E-594.9045642
TTCCAGC254.6474815E-794.9045642
ACGTGCC500.094.9045643
AATCTGC254.6474815E-794.9045642
CATACTG254.6474815E-794.9045641
GCGATGC254.6474815E-794.9045642
GCCTAGC201.7523202E-594.9045642
AACCTGC201.7523202E-594.9045642
GACGATG201.7523202E-594.9045641
GGATTGC254.6474815E-794.9045642
GATTCTG450.094.9045641
GCTAATG254.6474815E-794.9045641
Produced by FastQC (version 0.11.4)