FastQCFastQC Report
Tue 11 Oct 2022
A740_Gastroc_S29_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA740_Gastroc_S29_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876075
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG14070.1606026881260166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA650.095.040921
TCGTTTA650.095.040921
TTGTGGA450.095.040921
GGTGGGA450.095.040921
GTTTTTA900.095.040921
GGATGGA254.6079003E-795.040911
TCGGGCA254.6079003E-795.040911
TTCGTGA254.6079003E-795.040911
GCGTTTA254.6079003E-795.040911
AGCGGTA254.6079003E-795.040911
CGTAGGA254.6079003E-795.040911
TTTGGGA500.095.040911
GGGTTTA500.095.040911
TATCGCA254.6079003E-795.040911
GGAGGGA500.095.040911
TCACGAA201.7398743E-595.04091
GTGTTGA409.094947E-1295.04091
GTCATGA353.2923708E-1095.04091
GTTTGCA201.7398743E-595.04091
AGCGTGA156.606081E-495.04091
Produced by FastQC (version 0.11.4)