FastQCFastQC Report
Tue 11 Oct 2022
A739_SpCord_S44_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_SpCord_S44_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062042
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATCC156.645417E-494.9004752
AGCAATG201.7528679E-594.9004751
GGCAGGA450.094.9004753
GCCTTGC409.094947E-1294.9004752
GTACCAC156.645417E-494.9004754
TCCGCCC800.094.9004754
GAATCTG409.094947E-1294.9004751
GATCCAC450.094.9004754
CGACATG600.094.9004757
AGTGGAC7600.094.9004754
ATCGTGC409.094947E-1294.9004752
AACGGAC900.094.9004754
AATGCAC9550.094.9004754
GCCTGCA550.094.9004753
GATGCAC4800.094.9004754
CGAGCCC550.094.9004754
CATGTGC409.094947E-1294.9004752
CATAAGC600.094.9004752
GCAATGC450.094.9004752
GGACCTG301.2409146E-894.9004751
Produced by FastQC (version 0.11.4)