FastQCFastQC Report
Tue 11 Oct 2022
A739_SpCord_S44_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_SpCord_S44_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062042
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG17780.16741334146860481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTTA1400.095.027521
AGCGTTA254.612466E-795.027521
CTTCGCA156.610171E-495.027521
CAGCGTA201.7412623E-595.027521
ATGGGGA201.7412623E-595.027521
ATGGGCA254.612466E-795.027521
TCGTTTA550.095.027521
TCTTGGA254.612466E-795.027521
CCGGGTA254.612466E-795.027521
GGTACGA201.7412623E-595.027521
GAAGCGA500.095.027521
ACCGGGA201.7412623E-595.027521
GCAAGCA156.610171E-495.027521
GATGTGA450.095.027521
TTGTGTA700.095.027521
GGTCGCA156.610171E-495.027521
GCGGTTA156.610171E-495.027521
CGTGCTA201.7412623E-595.027521
GCGGTGA254.612466E-795.027521
GGATGCA201.7412623E-595.027521
Produced by FastQC (version 0.11.4)