FastQCFastQC Report
Tue 11 Oct 2022
A739_Liver_S38_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_Liver_S38_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396368
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCAC950.094.8977364
TAGCGGC156.6466216E-494.8977362
GAATCCT2000.094.8977367
GCGCCAT201.7533128E-594.8977366
ATTCGAC156.6466216E-494.8977364
ATGTCTC1250.094.8977365
GCGTTGC201.7533128E-594.8977362
ATACTGC950.094.8977362
TGCATGC800.094.8977362
GTCACAC254.6509194E-794.8977366
AGCGAGC156.6466216E-494.8977362
CGAGCAC1700.094.8977364
AGAGGTA201.7533128E-594.8977363
ATGCCGC500.094.8977362
CGTCCGC156.6466216E-494.8977362
GCACTTG156.6466216E-494.8977361
GGCTCGA156.6466216E-494.8977366
GGCTCCA156.6466216E-494.8977366
CGCCATG254.6509194E-794.8977367
GTTGCAC9550.094.8977364
Produced by FastQC (version 0.11.4)