FastQCFastQC Report
Tue 11 Oct 2022
A739_Liver_S38_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_Liver_S38_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396368
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG29980.2146998498963024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTA409.094947E-1295.032081
CGCGGGA201.741041E-595.032081
GGTGCTA409.094947E-1295.032081
GCGCTTA409.094947E-1295.032081
GGTACGA254.611902E-795.0320741
GTTCTTA650.095.0320741
GGTCGTA301.229273E-895.0320741
TGTGGCA254.611902E-795.0320741
CGCTGTA254.611902E-795.0320741
AGGCGGA156.609352E-495.0320741
GGTGCAA600.095.0320741
TTTGCGA254.611902E-795.0320741
CGATGGA254.611902E-795.0320741
CGATGAA301.229273E-895.0320741
TAGGCGA156.609352E-495.0320741
GTTGGCA254.611902E-795.0320741
TCGCGCA156.609352E-495.0320741
TTGGGGA500.095.0320741
TGTTGCG254.6197783E-795.0048458
CATGCGC201.743521E-595.004849
Produced by FastQC (version 0.11.4)