FastQCFastQC Report
Tue 11 Oct 2022
A739_FrCx_S50_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_FrCx_S50_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219473
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACA19400.094.899275
CGGCCCA2750.094.899265
ATGTGCA2700.094.899263
AGTATGC1550.094.899262
GGCCATT26400.094.899267
AGGCCAT8950.094.899266
TGCGCCC1550.094.899264
GATGCAC5100.094.899264
ATCCTGC254.650119E-794.8992542
CAGGTGC409.094947E-1294.8992542
ATGGGCT4100.094.8992545
AGACTGC700.094.8992542
GGAGCCC301.2410965E-894.8992544
GCGGTTG156.6459936E-494.8992541
GGATGCC550.094.8992543
GAACTGC650.094.8992542
GACCGCC156.6459936E-494.8992543
TCCCTGC201.7530838E-594.8992542
GTGATGC900.094.8992542
TTCGTGC550.094.8992542
Produced by FastQC (version 0.11.4)