FastQCFastQC Report
Tue 11 Oct 2022
A739_FrCx_S50_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_FrCx_S50_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219473
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG20360.16695736600974356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA650.095.030291
GGTACGA201.7411148E-595.030291
GATGTGA409.094947E-1295.030291
GGTGTGA500.095.030291
GTTTCGA450.095.030291
CGCGGGA156.609643E-495.030291
GCGGTGA201.7411148E-595.030291
CCGATGA156.609643E-495.030291
GCGCGTA201.7411148E-595.030291
GCGCGCA301.229273E-895.030291
GGCACGA254.6120658E-795.030291
TAGGTCA450.095.030291
TATGGGA600.095.030291
TGTGGCA301.229273E-895.030291
TGGCGGA301.229273E-895.030291
GGTCCGA156.609643E-495.030291
TTCCGTA201.7411148E-595.030291
GCGTTTA409.094947E-1295.030291
AGGCGGA254.6120658E-795.030291
CGTTCGA156.609643E-495.030291
Produced by FastQC (version 0.11.4)