FastQCFastQC Report
Tue 11 Oct 2022
A739_DRG_S56_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_DRG_S56_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848169
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC156.647574E-494.896892
ATGCGAC301.242006E-894.896894
AGGTCCG156.647574E-494.896891
CAGCGGA201.7537059E-594.896883
AGTCCCG409.094947E-1294.896881
GATCCTG500.094.896881
GGCTTTG1450.094.896881
GAGCCGC201.7537059E-594.896882
GCGGAGC500.094.896882
AGGATGC750.094.896882
AGGGATG500.094.896881
AAGGGCT4050.094.896875
GGCTCTG550.094.896871
GGCTCAA409.094947E-1294.895226
GGACATT48400.094.895217
GGACACT16500.094.607667
ACACCGC110550.094.42319
CCACCGC82850.094.379789
GCACACC62900.094.367177
GGGACAT50450.094.330926
Produced by FastQC (version 0.11.4)