FastQCFastQC Report
Tue 11 Oct 2022
A739_DRG_S56_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA739_DRG_S56_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848169
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG48540.17042528024144635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA800.095.039411
GCGGTTA600.095.039411
GGTTCGA600.095.039411
TGTCGCG254.6201967E-795.007698
TTAGGAC900.094.999352
AACCGAC353.3105607E-1094.999352
TGCTTAC650.094.999352
GCGGTAC353.3105607E-1094.999352
GTTCGAT353.3105607E-1094.999352
GATGGAT450.094.999352
TGCGAAC450.094.999352
TTGCGAC353.3105607E-1094.999352
ATGCGAC201.7443339E-594.999352
AGGGGAT409.094947E-1294.999352
TTCGCAT156.6191197E-494.999352
GTACGAT156.6191197E-494.999352
GGTTTAC1100.094.9993442
AGGGTAC550.094.9993442
TGCGTAC254.622616E-794.9993442
GAATGGC1600.094.9493266
Produced by FastQC (version 0.11.4)