FastQCFastQC Report
Tue 11 Oct 2022
A738_SpCord_S45_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA738_SpCord_S45_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661506
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC156.646176E-494.893733
ACAACTG156.646176E-494.893731
GTCATAC156.646176E-494.893732
ATGGGGA1400.094.893733
CGTGGGA353.3287506E-1094.893733
GAGTGCC254.6493733E-794.893733
AGCCGCC156.646176E-494.893733
TCTGAGC156.646176E-494.893732
AATCCGC156.646176E-494.893732
GCCCATA3800.094.893737
CATATGC550.094.893732
GCGACAC254.6493733E-794.893736
ATCTAGC301.2407327E-894.893732
AGTCAGC254.6493733E-794.893732
TCCTGCC353.3287506E-1094.893733
CAGTTGC254.6493733E-794.893732
ATGTGGA1900.094.893733
GGCGTGC156.646176E-494.893732
ATCAGGA550.094.893733
GAGTAGC301.2407327E-894.893732
Produced by FastQC (version 0.11.4)