FastQCFastQC Report
Tue 11 Oct 2022
A738_SpCord_S45_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA738_SpCord_S45_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661506
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG9760.1475421235786221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTTA950.095.03141
TATGTTA650.095.03141
TCTGCAA201.740422E-595.0313951
TTGATGA409.094947E-1295.0313951
GTTTGCA156.6079875E-495.0313951
AGCGTTA409.094947E-1295.0313951
CGGGTGA156.6079875E-495.0313951
ATGGGGA201.740422E-595.0313951
TCGTTTA450.095.0313951
GGTACGA201.740422E-595.0313951
GTACCGA201.740422E-595.0313951
GATGTGA201.740422E-595.0313951
AGTTTGA201.740422E-595.0313951
GGTGTGA353.2923708E-1095.0313951
GGGCTTA254.609392E-795.0313951
GGTCGTA156.6079875E-495.0313951
ATAGGGA254.609392E-795.0313951
CGAGTAA156.6079875E-495.0313951
GCGCGGA156.6079875E-495.0313951
GCGCGAA201.740422E-595.0313951
Produced by FastQC (version 0.11.4)