FastQCFastQC Report
Tue 11 Oct 2022
A738_Liver_S39_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA738_Liver_S39_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264036
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGC850.094.895892
GTACCTG201.7534183E-594.895891
GCGGTCT201.7534183E-594.895894
ATTCGAC301.2414603E-894.895894
ATAGAGC156.6469883E-494.895892
GGGAGCA156.6469883E-494.895893
ACGATGC550.094.895892
GTACTGC450.094.895892
AGCGATG201.7534183E-594.895891
TGCCTGC156.6469883E-494.895892
GGAGTGC409.094947E-1294.895892
ATGACTC409.094947E-1294.895895
CGGTGCC450.094.895893
ATGACGC201.7534183E-594.895892
GATGCCC2100.094.895894
GACGCAC450.094.895894
GACTGCC156.6469883E-494.895893
TCCTTGC800.094.895892
TTCTGCG156.6469883E-494.895891
GTCATGC500.094.895882
Produced by FastQC (version 0.11.4)