FastQCFastQC Report
Tue 11 Oct 2022
A738_Liver_S39_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA738_Liver_S39_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264036
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG21720.17183054912992984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGA353.2923708E-1095.023961
GTTTCGA409.094947E-1295.023961
CGTGCTA156.611451E-495.023961
TGGGTTA950.095.023961
TGTGGCA156.611451E-495.023961
TAGCGGA254.613994E-795.023961
TGGCGCA301.2300006E-895.023961
GGTCCGA201.7417158E-595.023961
TTCGTTA409.094947E-1295.023961
AGGGTTA450.095.023961
AAGCGTA409.094947E-1295.023961
TTCCGGA156.611451E-495.023961
GTAGGGA301.2300006E-895.023961
AAGCGGA254.613994E-795.023961
TCGATTA201.7417158E-595.023961
AGGCGGA301.2300006E-895.023961
CGGGGCA301.2300006E-895.023961
TTTGGTA600.095.023961
TTTGGGA600.095.023961
GTTCCGA156.611451E-495.023961
Produced by FastQC (version 0.11.4)