FastQCFastQC Report
Tue 11 Oct 2022
A738_FrCx_S51_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA738_FrCx_S51_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897680
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAC2200.094.8977364
GCACACC12200.094.8977367
AACATGC550.094.8977362
GTAAAGC550.094.8977362
ATGGGGA1300.094.897733
GGGGACT156.645843E-494.897733
AACCGCC301.2409146E-894.897733
GCCTTGC156.645843E-494.897732
AATTTGC950.094.897732
CATATGC900.094.897732
GCGACAC156.645843E-494.897736
ATCTAGC600.094.897732
GGCACTA156.645843E-494.897738
CAGTTGC600.094.897732
GGGTAGC156.645843E-494.897732
GCCGGGA156.645843E-494.897733
GCTGCAC254.6495552E-794.897734
CATCTGC156.645843E-494.897732
ATATGCC1500.094.897733
TGTGCAC8050.094.897734
Produced by FastQC (version 0.11.4)