FastQCFastQC Report
Tue 11 Oct 2022
A738_DRG_S57_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA738_DRG_S57_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363726
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC156.649388E-494.890754
TGGCGAC500.094.890754
CGATTCG156.649388E-494.890751
GACCCTG201.7543158E-594.890751
ATCCGCC353.3287506E-1094.890753
CGCTCTG254.6543937E-794.890751
GCCTGCG156.649388E-494.890751
CGCTAGC353.3287506E-1094.890752
CGCTACC156.649388E-494.890752
CAGGCGC156.649388E-494.890752
CAGGAGC156.649388E-494.890752
AGCGAGC353.3287506E-1094.890752
GATGCCG201.7543158E-594.890751
ACGTCCG201.7543158E-594.890751
GCGGAGC500.094.890752
GACGCGC201.7543158E-594.890752
ATCATGC2300.094.890752
GAGGCCT600.094.890755
GCGAGCC700.094.890753
CATGATG550.094.890741
Produced by FastQC (version 0.11.4)