FastQCFastQC Report
Tue 11 Oct 2022
A738_DRG_S57_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA738_DRG_S57_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363726
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG42440.12616961072334668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCA850.095.040281
GAAGGGA850.095.040281
GAAGCGA301.2290911E-895.0402761
GGTCCGA500.095.0402761
GATGGTA1500.095.0402761
AGTTGCG156.617287E-495.006357
TTAGGAC800.094.996472
GTAGGAC800.094.996472
GTGCTAT409.094947E-1294.996472
GATTCAT650.094.996472
AGGTCAT156.620026E-494.996462
AGCGTAC600.094.996462
GCGGTAC500.094.996462
TAGGTAT850.094.996462
ATGCTAC600.094.996462
AAGCGAC353.3105607E-1094.996462
TGTGCAT500.094.996462
GGGGTAC450.094.996462
TTTGGAC950.094.996462
GTCCGAC353.3105607E-1094.996462
Produced by FastQC (version 0.11.4)