FastQCFastQC Report
Tue 11 Oct 2022
A737_SpCord_S46_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_SpCord_S46_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591958
Sequences flagged as poor quality0
Sequence length101
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAA254.6496643E-794.8906256
TCACCGC254.6496643E-794.8906259
CGCGGCC500.094.8906254
ATCACAT254.6496643E-794.8906256
ATACTGC254.6496643E-794.8906252
GGGACAT7900.094.8906256
GTCACCG254.6496643E-794.8906258
GCGGGCA254.6496643E-794.8906253
CTTCTGC254.6496643E-794.8906252
CATGTGC500.094.8906252
TCCCCAC500.094.8906256
GACTGCA254.6496643E-794.8906253
TTCTGCA1000.094.8906253
ATTCTGC500.094.8906252
GTCGGAC254.6496643E-794.8906254
TCCGGAC1200.094.890624
AGACTGC156.6466944E-494.890622
GGGGACA6300.094.890625
AACCGGA156.6466944E-494.890623
GCCCATC3300.094.890627
Produced by FastQC (version 0.11.4)