FastQCFastQC Report
Tue 11 Oct 2022
A737_SpCord_S46_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_SpCord_S46_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591958
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG9310.1572746715138574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCA156.6056853E-495.038471
TTTAGCA156.6056853E-495.038471
CATGGGA201.7396269E-595.038471
CGGGTCA156.6056853E-495.038471
CTATGGA201.7396269E-595.038471
AATCCGA156.6056853E-495.038471
TCGTTCA156.6056853E-495.038471
GGTACGA254.6067544E-795.038471
GTACCGA156.6056853E-495.038471
GAAGCGA201.7396269E-595.038471
CCGGGAA156.6056853E-495.038471
TTGTGTA450.095.038471
GGATGTA301.22745405E-895.038471
GTTTCGA156.6056853E-495.038471
ACGTTTA156.6056853E-495.038471
GCGGTTA201.7396269E-595.038471
ACTTGTA201.7396269E-595.038471
GGATGGA201.7396269E-595.038471
ACGTTGA156.6056853E-495.038471
CCGATGA156.6056853E-495.038471
Produced by FastQC (version 0.11.4)