FastQCFastQC Report
Tue 11 Oct 2022
A737_Liver_S40_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_Liver_S40_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306706
Sequences flagged as poor quality0
Sequence length101
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC450.094.891264
GGAGCCC450.094.891264
GGATGCA450.094.891263
GGCCCAT5150.094.891266
GGACATT23950.094.891267
ATTGCAC9450.094.891264
GAGGACT201.7538658E-594.891254
GCGACAT201.7538658E-594.891256
ACACCCG201.7538658E-594.891251
GGCGTCT156.64833E-494.891254
GCTGCGG201.7538658E-594.891252
GCTGACT156.64833E-494.891254
ATCGTGC156.64833E-494.891252
GCCGCCC301.242006E-894.891254
GATGCAC3100.094.891254
GAACCGT156.64833E-494.891252
GATCTGC156.64833E-494.891252
GCAGCAC1350.094.891254
ATGCCGC201.7538658E-594.891252
ATTCTGC800.094.891252
Produced by FastQC (version 0.11.4)