FastQCFastQC Report
Tue 11 Oct 2022
A737_Liver_S40_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA737_Liver_S40_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306706
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG22240.1701989582966635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA600.095.038081
GAGGGCA156.6075975E-495.038081
GAAGGGA600.095.038081
GATGTGA353.2923708E-1095.038071
GCGGTTA201.7404538E-595.038071
GCTTCGA201.7404538E-595.038071
GGGCGGA353.2923708E-1095.038071
TAGGGTA450.095.038071
CGGTGCA201.7404538E-595.038071
TGTGTCA450.095.038071
TTTGTGA1250.095.038061
ATAGGCT3400.095.008967
AGTGGCG1350.095.008967
ATTTTCT3600.095.008967
AGTTTAC600.094.9907762
GAATGAT201.744761E-594.9907762
GCGGTAC156.620685E-494.9907762
TAGGTAC301.232911E-894.9907762
AAGCGAC156.620685E-494.9907762
CGTTCAT156.620685E-494.9907762
Produced by FastQC (version 0.11.4)